Journal: The Journal of cell biology

Article Title: Architecture of the cortical actomyosin network driving apical constriction in C. elegans.

doi: 10.1083/jcb.202302102

Figure Lengend Snippet: Figure 1. Models of apical constriction. (A) Illustration of apical constriction resulting in tissue morphogenesis. (B) Three of the models of actomyosin architecture observed in apically constricting cells. Orange shading represents the regions of localized myosin-based force generation. (C) Maximum-intensity projections of 10 planes spanning 5 μm total Z-depth depicting C. elegans gastrulation from a ventral view with plasma membranes fluorescently labeled (mex- 5p::mScarlet-I::PH). Ea and Ep are pseudocolored to visualize their internalization over time. L, R, A, and P indicate left, right, anterior, and posterior, re- spectively. Scale bar: 5 µm.

Article Snippet: The single-copy germline optimized LifeAct transgene was inserted into a neutral site on chromosome II (ttTi5605) using a Zhang et al. Journal of Cell Biology 6 of 9 Actomyosin architecture driving apical constriction https://doi.org/10.1083/jcb.202302102 D ow nloaded from http://rupress.org/jcb/article-pdf/222/9/e202302102/1453759/jcb_202302102.pdf by Indonesia user on 13 January 2024 repair template also containing a self-excising cassette with selectable markers to facilitate screening (Dickinson et al., 2015) paired with a plasmid encoding Cas9 and sgRNA (pDD122, #47550; AddGene).

Techniques: Clinical Proteomics, Labeling

Journal: The Journal of cell biology

Article Title: Architecture of the cortical actomyosin network driving apical constriction in C. elegans.

doi: 10.1083/jcb.202302102

Figure Lengend Snippet: Figure 2. NMY-2 exhibits punctate localization distributed throughout the apical cortex during apical constriction. (A) Micrographs from a time-lapse movie depicting dynamic localization of mNG::NMY-2 over time from a ventral view. NMY-2 is covisualized with mScarlet-I::PH, which labels the plasma membrane. Yellow arrowheads point to Ea and Ep cells. (B) An enlarged micrograph from panel A to demonstrate how line scan (yellow line) measurements were collected. (C) Plots depicting fluorescence intensity of mNG::NMY-2, normalized to the mean intensity, along the left–right axis of Ea (n = 11 embryos). Solid lines indicate the mean, and shaded areas indicate the mean ± SD. Time represents minutes following the birth of the neighboring MSxx cells. Scale bars: 5 µm.

Article Snippet: The single-copy germline optimized LifeAct transgene was inserted into a neutral site on chromosome II (ttTi5605) using a Zhang et al. Journal of Cell Biology 6 of 9 Actomyosin architecture driving apical constriction https://doi.org/10.1083/jcb.202302102 D ow nloaded from http://rupress.org/jcb/article-pdf/222/9/e202302102/1453759/jcb_202302102.pdf by Indonesia user on 13 January 2024 repair template also containing a self-excising cassette with selectable markers to facilitate screening (Dickinson et al., 2015) paired with a plasmid encoding Cas9 and sgRNA (pDD122, #47550; AddGene).

Techniques: Clinical Proteomics, Membrane, Fluorescence

Journal: The Journal of cell biology

Article Title: Architecture of the cortical actomyosin network driving apical constriction in C. elegans.

doi: 10.1083/jcb.202302102

Figure Lengend Snippet: Figure 5. Actomyosin architectures driving apical constriction in diverse biological contexts. Illustrations depicting actin filament polarization and localization of myosin and myosin-activating kinase in the Drosophila ventral furrow, C. elegans gastrulation, and other contexts of apical constriction in Xenopus, chick, and mouse (see Table S1 for additional details).

Article Snippet: The single-copy germline optimized LifeAct transgene was inserted into a neutral site on chromosome II (ttTi5605) using a Zhang et al. Journal of Cell Biology 6 of 9 Actomyosin architecture driving apical constriction https://doi.org/10.1083/jcb.202302102 D ow nloaded from http://rupress.org/jcb/article-pdf/222/9/e202302102/1453759/jcb_202302102.pdf by Indonesia user on 13 January 2024 repair template also containing a self-excising cassette with selectable markers to facilitate screening (Dickinson et al., 2015) paired with a plasmid encoding Cas9 and sgRNA (pDD122, #47550; AddGene).

Techniques:

Journal: Developmental cell

Article Title: Comprehensive endogenous tagging of basement membrane components reveals dynamic movement within the matrix scaffolding

doi: 10.1016/j.devcel.2020.05.022

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: 2017 NK2318 ten-1::LL::mNG CRISPR knock-in; qy56 This study NK2502 ddr-1::LL::mNG CRISPR knock-in; qy43 This study NK2456 ddr-2::LL::mNG CRISPR knock-in; qy44 This study NK2457 ptp-3::LL::mNG CRISPR knock-in; qy47 This study NK2477 sdn-1::LL::mNG CRISPR knock-in; qy29 This study NK2413 dgn-2::LL::mNG CRISPR knock-in; qy 82 This study NK2587 gon-1::LL::mNG CRISPR knock-in; qy45 This study NK2590 mig-17::mNG::3xFlag; shc19 Ji et al. 2019 FDU1506 Oligonucleotides Table S2 Recombinant DNA pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA) Addgene 47550 mNG-C1^SEC^linker This study mNG-SEC-LL mNG-C1^SEC^bothlink This study mNG-SEC-bothlink mEos2-C1^SEC This study mEos2-SEC L4440 RNAi empty vector control Addgene 1654 mig-6 RNAi This study / Ahringer mig-6 RNAi Software and Algorithms Fiji Schindelin et al., 2012 Image J 2.0.0-rc-69/1.52p, http://fiji.sc/ Photoshop Adobe Version CC 2018 Imaris Bitplane Version 9 Illustrator Adobe Version CC 2018 R GNU Project Open Source μManager Edelstein et al., 2010 Version 1.4, https://micromanager.org/ iLas Biovision V.1.2.3 MetaMorph Molecular Devices 7.10.3.279 Excel Microsoft Version 15.39 GraphPad Prism GraphPad Version 7 Open in a separate window KEY RESOURCES TABLE In vivo visualization of 29 basement membrane (BM) proteins fused with mNeonGreen Embryonic BMs share a common template but diversify greatly during development Stable BM proteins form a scaffold that supports movement of dynamic BM components Papilin promotes local BM collagen removal by limiting GON-1 protease localization

Techniques: Virus, Recombinant, Mutagenesis, CRISPR, Knock-In, Clinical Proteomics, Membrane, Plasmid Preparation, Control, Software